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. 2018 Mar 28;8:5314. doi: 10.1038/s41598-018-22488-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of total RPM at miRNA arms and mature tRNA loci. Separately for each sample, we summed the RPM values of each isomiR that arises from a specific locus and assigned the resulting value to that locus. There were 628 loci that produced isomiRs in various combinations in the 526 samples. At most 20 of the miRNA-arms exhibiting differential abundances are shown in each case, if available. (A) PRAD vs. normal (all patients). (B) PRAD vs. normal (Wh patients only). (C) PRAD vs. normal (B/Aa patients only). (D) PRAD from Wh patients vs. PRAD from B/Aa patients. (E–H) counterpart plots to (A–D) for isoacceptors. Analogously, we summed the RPM values of each tRF that arises from a given isoacceptor, and assigned the resulting value to the isoacceptor. There were 47 isoacceptors that produce tRFs in various combinations in the 526 samples. At most 20 of the isoacceptors exhibiting differential abundances are shown in each case, if available.