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. 2018 Mar 28;8:5336. doi: 10.1038/s41598-018-23549-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) and (B) Blast T cells from the indicated mice were stimulated with increasing concentration of anti-CD3 coated antibody during the indicated time. Cell lysates were analyzed by immunoblotting for Cish, PLC-γ1 and Zap-70 (Loading control). Numbers below the band indicate PLCγ1 expression quantification. (C) Quantification of PLC-γ1 expression from 3 experiments. Non-stimulated conditions represent 100% of PLC-γ1 expression. Bars show mean + SEM out of 3 independent experiments. Statistical significance was determined by two-tailed Student’s t test. *P ≤ 0.05. (NS = Non-stimulated).