Compound 38u inhibits invasion in vitro. (A) Effect of compound 38u on cell proliferation, invasion, and migration of MDA-MB-231 cells. Cell proliferation was determined by MTT assay, following incubation with the compound. (B) Effect of compound 38u on cell proliferation, invasion, and migration of MDA-MB-435 cells. Cell proliferation was determined by MTT assay, following incubation with the compound. (C) Effect of compound 38u on AEP activity in MDA-MB-231 and MDA-MB-435 cells. AEP activity were examined with lysate of cells treated by 38u (D) Effect of compound 38u on invasion of MDA-MB-231 and MDA-MB-435 cells treated by control and AEP siRNA.