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. 2018 Mar 28;8:5301. doi: 10.1038/s41598-018-23591-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Example of event. (a–e) The image sequence shows several raw frames of a video (Supplementary Movie S2) centered on one capillary acquired with our prototype on one of the patients at baseline. The dark loop corresponds to the capillary vessel filled with RBCs that absorbs light at the illumination wavelength. An optical absorption gap in the microcirculation, approximately the same size as the capillary width (~15 µm) can be observed flowing through the arterial limb of the capillary (black triangular arrowheads). Frame numbers are labeled at the top right corner. The frame rate was 60 FPS and the exposure time 16.7 ms. The contrast was adjusted for the ROI shown.