Table 2.

The PCR reaction system.

Reaction system components	Volume (μL)
SYBR premis Ex Taq (Tli RNase Plus) (2×)	5.0
PCR forward primer (10 μmol/L)	0.2
PCR reverse primer (10 μmol/L)	0.2
ROX reference Dye II (50×)	0.2
DNA	1.0
dH2O	3.4
Total volume	10.0