Fig. 2.

VASP knockdown suppresses the growth of 3D cancer spheroids by reducing P-FAK and YAP1/TAZ protein levels. a VASP knockdown in two human CRC cell lines significantly reduced the size of cancer spheroids. *p < 0.05 by t-test, n > 50 per group; Bar, 100 µm. b VASP knockdown in two PDAC cancer cell lines significantly reduced the size of cancer spheroids. *p < 0.05 by t-test, n > 50 per group; Bar, 100 µm. c Cancer spheroids were harvested for WB. VASP knockdown in 4 cell lines consistently reduced phosphorylation of FAK and Src and protein levels of YAP1/TAZ. The effect of VASP knockdown on phosphorylation of Akt or Erk was not consistent and it did not influence the total FAK and Src protein levels. Quantitative data of HCT116 are shown on the bottom. *p < 0.05, by t-test, n = 3. Samples derived from the same experiment and gels/blots were processed in parallel. Error bar: S.D