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. 2018 Mar 21;9:360. doi: 10.3389/fpls.2018.00360

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Copyright © 2018 Negrel, Halter, Wiedemann-Merdinoglu, Rustenholz, Merdinoglu, Hugueney and Baltenweck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of ceramides in sporangia extracts. (A) EIC of sporangia extracts for m/z 236.2371 (±2 ppm), a characteristic ion of C16:1 ceramides. Peaks corresponding to Plasmopara-specific ions (Table 1) are numbered from 1 to 4 and retention times are indicated. Peak 1: M492T96 = Cer(d16:1/16:0); peak 2: M521T125 = Cer(d16:1/18:0); peak 3: M549T166 = Cer(d16:1/20:0); peak 4: Cer(d16:1/22:0). Identity of Cer(d16:1/16:0) was confirmed by using the corresponding standard. (B) Mass spectrum of putative Cer(d16:1/18:0) (M521T125) from P. viticola. The fragmentation giving rise to the main fragments is indicated. A detailed fragmentation is given in Supplementary Figure 3.