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. 2018 Mar 21;9:360. doi: 10.3389/fpls.2018.00360

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Copyright © 2018 Negrel, Halter, Wiedemann-Merdinoglu, Rustenholz, Merdinoglu, Hugueney and Baltenweck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Markers of P. viticola in downy mildew-infected grapevine leaves before sporulation. Analysis of MeOH/CHCl₃ extracts presenting the sum of the EIC for m/z of the characterized lipids of P. viticola (±2 ppm): 303.2316 + 305.2474 + 377.2683 + 379.2839 + 492.4773 + 520.5084 + 548.5399 + 576.5713 + 661.4825 + 665.5134 + 945.6964. (A) Typical pattern obtained from downy mildew-infected leaves (Muscat Ottonel) 4 days post infection; (B) control leaves; (C) sporangia of P. viticola. For easier comparison, infected (A) and control leaves (B) are presented at the same intensity scale (3.10⁶ arbitrary units). Peaks corresponding to lipids of P. viticola are numbered from 1 to 6 and the corresponding retention times are indicated. Peak 1: AA, EPA, AG, EPG; peak 2: DAG, DEPG, Cer(d16:1/16:0); peak 3: Cer(d16:1/18:0); peak 4: Cer(d16:1/20:0); peak 5: Cer(d16:1/22:0); peak 6: TEPG.