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. 2018 Jan 11;7(1):10. doi: 10.3390/biology7010010

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RAD51 immuno-positive signals. DAPI column shows DAPI nuclear staining (blue); Rad51 column shows the same DAPI-stained cells expressing Rad51 protein (green). Ozone treatment was able to increase Rad51 immuno-profiles (red arrows). CTR + O₂ = oxygen-treated rats; CTR + O₃ = ozone-treated rats; STZ + O₂ = oxygen-treated STZ rats; STZ + O₃ = ozone-treated STZ rats. Negative CTR: Negative control (without primary antibody) for staining in order to confirm antibody specificity.