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. 2018 Jan 24;8(1):10. doi: 10.3390/bios8010010

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Schematic presentation of six distinct assay formats for colorimetric detection: (I) cAB + IL8 + gold-labeled dAB, (II) cAB + IL8 + carbon-labeled dAB, (III) cAB + IL8 + oxidized carbon-labeled dAB, (IV) cAB + IL8 + biot. dAB + neutravidin–carbon, (V) cAB + IL8 + biot. dAB + strp–gold, and (VI) cAB + IL8 + biot. dAB + strp-HRP + enzyme substrate. Slides treated via gold nanoparticles were further stained by silver (Ag). (b) Illustration of the antibody (in orange) coupling process using gold (red) and carbon (black) nanoparticles.

(a) Schematic presentation of six distinct assay formats for colorimetric detection: (I) cAB + IL8 + gold-labeled dAB, (II) cAB + IL8 + carbon-labeled dAB, (III) cAB + IL8 + oxidized carbon-labeled dAB, (IV) cAB + IL8 + biot. dAB + neutravidin–carbon, (V) cAB + IL8 + biot. dAB + strp–gold, and (VI) cAB + IL8 + biot. dAB + strp-HRP + enzyme substrate. Slides treated via gold nanoparticles were further stained by silver (Ag). (b) Illustration of the antibody (in orange) coupling process using gold (red) and carbon (black) nanoparticles.