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. 2018 Feb 26;8(1):17. doi: 10.3390/bios8010017

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Chip-based LAMP POC diagnostics. Detection with fluorescent intercalating dye: excitation with Smartphone flashlight LED, emission intensity monitored with a Smartphone CCD camera. (A) Real-time fluorescence intensity curves as a function of amplification incubation time for four concentrations of Zika virus (0, 5, 50 and 500 PFU, plaque forming units). By establishing a threshold fluorescence level (e.g., time needed for the signal to reach half its saturation value), a threshold time for each concentration can be determined; (B) threshold time correlates inversely with the log of Zika concentration (PFU, plaque forming units), providing a calibration curve to estimate Zika concentration in sample. From [103].