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. 2018 Feb 26;8(1):17. doi: 10.3390/bios8010017

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Nuclemeter for Quantifying Nucleic Acids: Nuclemeter chip features an array of reaction-diffusion microconduits for isothermal amplification. The chamber and conduits are filled with LAMP amplification mix including an intercalating DNA green dye, primers, and hydroxypropyl-methyl-cellulose as a low-viscosity sieving matrix to reduce diffusivity. The target nucleic acid template is introduced at the column’s inlet. As isothermal amplification proceeds, the amplification products diffuse down the channel, such that the distance of the reaction front from the chamber X_F can be correlated with time and template number (copies). At any given time, the length of the fluorescent column can be calibrated against a ruler scale imprinted on the chip. (a) Cross-section of reaction-diffusion channel; (b) schematic of reaction front, (c) photo of nuclemeter chip; (d) Photos of reaction diffusion conduits at 0, 16, 32 and 56 min, and (e) Diffusion front X_F position (mm) as a function of time for three different sample (initial) template concentrations: 10⁴, 10⁵ and 10⁶ copies. From [115].

Nuclemeter for Quantifying Nucleic Acids: Nuclemeter chip features an array of reaction-diffusion microconduits for isothermal amplification. The chamber and conduits are filled with LAMP amplification mix including an intercalating DNA green dye, primers, and hydroxypropyl-methyl-cellulose as a low-viscosity sieving matrix to reduce diffusivity. The target nucleic acid template is introduced at the column’s inlet. As isothermal amplification proceeds, the amplification products diffuse down the channel, such that the distance of the reaction front from the chamber X_F can be correlated with time and template number (copies). At any given time, the length of the fluorescent column can be calibrated against a ruler scale imprinted on the chip. (a) Cross-section of reaction-diffusion channel; (b) schematic of reaction front, (c) photo of nuclemeter chip; (d) Photos of reaction diffusion conduits at 0, 16, 32 and 56 min, and (e) Diffusion front X_F position (mm) as a function of time for three different sample (initial) template concentrations: 10⁴, 10⁵ and 10⁶ copies. From [115].