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. 2018 Feb 26;8(1):17. doi: 10.3390/bios8010017

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Smart Cup: The Smart cup is a customized Thermos^® bottle that provides the inserted single-use chip with constant temperature heating. The Smart Cup has a built in mount for positioning a Smartphone CCD camera to monitor the reaction progress. Heating is due to an exothermic reaction of Mg-Fe powder initiated by addition of water. A phase-change material (PCM) maintains the chip at a constant ~65 °C temperature for about 60 min. (A) Cross-section of Smart Cup showing Mg-Fe alloy pouch that is activated by adding water to initiate heating, PCM, heat sink and slot holding microfluidic chip. Smartphone is held for optimal focus of Smartphone CCD camera on chip amplification chamber to measure fluorescence; (B) field unit and companion chip (inset). The smart cup can be optionally made of Styrofoam, making the entire system disposable. From [144].