Table 3.
Multidrug resistance of puromycin-resistant and unselected mouse cell lines without drug-resistance genes
| Relative resistance of
puromycin-resistant cells in
percent*
|
Resistance of unselected cells in
percent
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Cell | Puromycin (20 μg) | Puromycin (20 μg) + araC (3 μg) | Puromycin (20 μg) + colcemid (0.1 μg) | Puromycin (20 μg) + Actinomycin (0.3 μg) | Cell | Puromycin (2.5 μg, 5 μg) | AraC (0.6 μg, 3 μg) | Colcemid (0.1 μg) | Actinomycin D (0.15 μg) |
| 771-P20 | 100 | 50 | 70 | <1† | 771 | 7.5, 0 | 20, <1 | 20 | <1 |
| 886-P20 | 100 | 65 | 50 | <1† | 886 | 2, 0 | 75, <1 | 50 | <1 |
| 4B-P20 | 100 | 90 | 60 | <1† | 4b | 3, 0 | 90, <1 | 10 | 0 |
*
Final resistances against araC and colcemid were recorded 2–3 weeks after initiation of treatment (see text) and otherwise as in Table 2.
†
A few colonies were obtained if treatment was interrupted after 2–4 days.