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. 2017 Dec 23;7(1):3. doi: 10.3390/antibiotics7010003

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Copy numbers of secondary replicons in E. coli dependent on crtS. (A) Copy number of an ori2-based minichromosome in an E. coli strain without crtS (Strain NZ72) or with crtS-site insertion on the chromosome (Strain NZ140) was measured as inverse of the lag time for cells in medium with high concentrations of ampicillin (500 µg/ mL). Strains carrying an oriF-based replicon and the crtS (Strain NZ141) or no crtS on the chromosome (Strain NZ119) were used as control. Data are the mean of three biological replicates with the indicated standard deviations. (B) Copy number of an ori2-minichromosome (pMA568) analyzed by qPCR-based marker frequency analysis relative to oriC. Mean values of three biological replicates are shown with the respective standard deviations.