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. 2017 Dec 23;7(1):3. doi: 10.3390/antibiotics7010003

Figure 4.

Figure 4

Conjugation rate of extra replicons to oriC and ori2 E. coli strains. (A) Transfer by conjugation was tested for oriC-minichromosomes carrying a crtS site (pMA200) or no crtS (pMA308) into E. coli with chromosome replication based on oriC (MG1655) or ori2 (NZ90). (B) Conjugation of oriF-based replicons with crtS (pMA206) or without (pMA899) into E. coli with oriC (MG1655), ori2 (NZ90) or oriC at the native site and ori2 at an ectopic site (#15) [25,29]. Mean values of three biological replicates are shown in (A,B) with the actual numbers and standard deviations given in the table (C).