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. 2017 Dec 23;7(1):3. doi: 10.3390/antibiotics7010003

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of vibrepin on ori2-dependent replication. Lag time (time until OD = 0.1 was reached) is shown as the mean value of three replicates, with the respective standard deviations, in medium without vibrepin (blue) or with vibrepin (red). Vibrepin concentrations were 16 μg/mL for E. coli strains (A–B) and 1.6 μg/mL for V. cholerae strains (C). Analyzed E. coli strains replicated their chromosome based on oriC (MG1655) or ori1 (NZ90) (A) or carried an extra minireplicon with ori2 (pMA100), oriC (pMA106) or oriF (pMA129) [38] (B) as indicated. V. cholerae strains are the standard two-chromosome strain N16961 [6], an engineered derivative of N16961 with fused chromosomes (MCH1) [36] or a natural isolate with fused chromosomes (NSCV1) [37].