Table 1.

Possible molecular engineering strategies with potential application(s).

Modification	Property	Example	Reference
Truncation of full-length enzyme	Increased catalytic ability and solubility	CHAPk	Horgan et al. [19]
Fusion of EADs with CBDs of different endolysins	Increased catalytic ability and solubility	ClyS	Daniel et al. [20]
	Increased catalytic ability and broader lytic spectrum	SA2-E-Lyso-SH3b, SA2-E-LysK-SH3b	Schmelcher et al. [21]
	Thermostability	PlyGVE2CpCWB	Swift et al. [22]
Fusion of virion-associated lysin with CBD of endolysin	Increased efficacy	EC300	Proença et al. [23]
Endolysin fusion with OMP	Increased efficacy towards Gram-negative bacteria	OBPgp279, PVP-SE1g-146	Briers et al. [24]
Endolysin fusion with AMP	Increased efficacy towards Gram-negative bacteria	Art-175	Briers et al. [25]
Truncation and site-directed mutagenesis	AMP development	LysAB2	Peng et al. [26]

EAD: enzymatically-active domain; CBD: cell wall-binding domain; OMP: outer-membrane permeabilizer; AMP: antimicrobial peptide.