Table 1.
Comparisons between PCho and Cho colorimetric measurements, as well as the PCho measurement as inferred from the Cho measurement (inverse [Cho]), from both the control and the HC-3 reactions. Asterisks are shown in places where a calculation could not be made due to limitations in the sensitivity of the MBD method. Bars are shown where measurements were not made.
| Control Reaction (μM) | HC-3 Reaction (μM) | |||||||
|---|---|---|---|---|---|---|---|---|
| Minutes | [PCho] | Inverse [Cho] | [Cho] | [Cho] MS | [PCho] MS | [Pcholine] | [Cho] MS | [Pcho] MS |
| 0 | * | 72.6 | 927.4 | - | - | * | - | - |
| 20 | 284.3 | 270.4 | 729.6 | - | - | * | - | - |
| 40 | 374.8 | 367.7 | 632.3 | - | - | 258.5 | - | - |
| 60 | 451.7 | 445.2 | 554.8 | 521.1 | 422.2 | 284.9 | 741.1 | 295.2 |
| 80 | 514.4 | 502.5 | 497.5 | 478.7 | 540.9 | 325.5 | 693.1 | 326.2 |
| 100 | 551.7 | 579.2 | 420.8 | 462.6 | 549.5 | 354.3 | 668.9 | 375.3 |