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. 2018 Mar 18;7(1):25. doi: 10.3390/antibiotics7010025

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© 2018 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of nucleoside diphosphates on the phosphorolysis of the 5650 transcript. Phosphorolysis reactions were performed as described in [47], and reaction products were separated by gel electrophoresis. The top panel shows the results obtained with S. coelicolor PNPase and the bottom panel results using E. coli PNPase. Reactions were conducted in the presence of increasing concentrations of a mixture of ADP, CDP, UDP, and GDP (nucleoside diphosphates (NDPs)) as indicated. RP3 is the 5650 transcript, and RP4 represents the product obtained by complete digestion of the intergenic hairpin in RP3 by PNPase. Note that as PNPase is highly processive [48], no intermediates with mobilities between those of RP3 and RP4 were observed. Copyright © American Society for Microbiology (J. Bacteriol. 190, 2008, 98–106, DOI:10.1128/JB.00327-07) [47].