Figure 2.

Immunoblot analysis of capsular polysaccharides in TIGR4 and mutant strains. All strains were cultured in THY supplemented with fetal bovine serum (FBS) to mid-log phase. Total capsular polysaccharide (CPS) isolated from equal number of cells for each strain, and 3× dilutions were spotted onto a nitrocellulose membrane. Membranes were probed with rabbit anti-serotype 4 sera and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody. Membranes were developed with enhanced chemiluminiscence (ECL) detection and scanned using a ChemiDoc XRS+ with Image Lab software (Bio-Rad, Hercules, CA, USA). Data from representative immunoblot from two independent colonies for each strain are shown.