Table 1.

Sequences of primers used in this study.

Primer	Sequence * (5′→3′)	Experiment
cadAF1	AGCAAATATAAACCCGAGTAAAAA	Mutagenesis
cadAR1	CAGGTACCGCTTGTGACCTGGAACATC	Mutagenesis
cadAF2	CAGAGCTCGTTTCGGTTTGCGATTTT	Mutagenesis
cadAR2	GATCTTCCGTCCCTTGGAG	Mutagenesis
cadAF-XbaI	TTCCCCGGGCCGTGCGAAAATCATCGCC	Complementation
cadAR-SacI	ATTCGAGGAAGACAGAGGTGTACTATTC	Complementation
gyrBF	CCGTCCTGCTGTTGAGACC	qRT-PCR
gyrBR	GTGAAGACCACCTGAAACCTTG	qRT-PCR
potDF	AAACCTGAAAATGCTCTCCAAAATG	qRT-PCR
potDR	CCTTATCTTCCTTTGTTTCCTCTGG	qRT-PCR
cps4AF	TCAAGTCAAGTCAGAATACCGATTTG	qRT-PCR
cps4AR	TCAAAGACACTATTTAGGACAATGGC	qRT-PCR
speEF	TGCGGATGATTTCGTCTACAATG	qRT-PCR
speER	CCAGTTCAGGATAGAGGGTTAATAC	qRT-PCR
aguAF	GCTTAGTCCTGGTCGCAATC	qRT-PCR
aguAR	CTGGGGATCATTTTCGTCAT	qRT-PCR
lys9F	GGCTTGACTGCTCTTCTTGG	qRT-PCR
lys9R	AGTAAGAACCTGGCGCAGAA	qRT-PCR
nspCF	ATGTATTTGCGCCTGCTTTC	qRT-PCR
nspCR	TGGTGCACAAGGGTCATAGA	qRT-PCR

* underlined sequence complementary to Streptococcus pneumoniae TIGR4 chromosomal DNA. qRT-PCR: quantitative reverse transcription-PCR.