TABLE 2 .
Oligonucleotides used in this study
| Oligonucleotide | Purpose | Sequence |
|---|---|---|
| KL12 | F primer to amplify RK2 oriT from pJC8, with HindIII adapter | CCTAAGCTTTCGGTCTTGCCTTGCTCGTCGG |
| KL13 | R primer to amplify RK2 oriT from pJC8, with HindIII adapter | CCTAAGCTTGCGCTTTTCCGCTGCATAACCC |
| KL14 | F primer to amplify ermF-repA fragment from pAFD1, with EcoRI adapter | CCTGAATTCACTTTTGTGCAATGTTGAAGATTAGTAATTCTATTC |
| KL15 | R primer to amplify ermF-repA fragment from pAFD1, with EcoRI adapter | CCTGAATTCATAACAGCCGGTGACAGCCGGC |
| KL16 | Primer walking ermF-repA fragment | GTTCAACCAAAGCTGTGTCGTTTTCAATAGC |
| KL33 | Primer walking ermF-repA fragment | CAGGTATGCCAAACGTGGTTCTAAAAATGC |
| KL42 | Primer walking ermF-repA fragment | GGAACTGCAAAATTCCTAAAATCACAACC |
| KL43 | Primer walking ermF-repA fragment | CAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGG |
| KL45 | Primer walking ermF-repA fragment | AACAGACAAAGCCGTTTATAAAGGACTTGC |
| KL46 | Primer walking ermF-repA fragment | GTCAGCAACAAAGGTAGTACTTTATTATCG |
| KL61 | F primer for B. thetaiotaomicron anSME ORF (BT_0238) | ATGAAAGCAACAACTTATGCACCTTTTGCCAAACC |
| KL62 | R primer for B. thetaiotaomicron anSME ORF (BT_0238) | TTAATATTCTATTTTTAAACTTCCGTCTTTTAGTGCTTTC |
| KL63 | F primer for 300 bp upstream of B. thetaiotaomicron anSME ORF | TCTCCATCCCTCAAAGTCTTCAGATATAACATTTTTCC |
| KL65 | R primer for 300 bp downstream of B. thetaiotaomicron anSME ORF | TAACCGCAGTGATGGTTAGTCAGGATCAAGC |
| KL67 | Sequence anSME PCR product from clone CLGM3_chuR5 | AAGCGGACGCATCAGCGTTTCTCCACC |
| KL69 | Sequence anSME PCR product from clone CLGM3_chuR5 | TCTATTTGCCTGCAACGGAGAATGTCC |