Table 5.

Normalized fluorescence levels (ΔRn values) obtained for SNP103 from the 20-fold dilutions using the three chemistries

DNA input (ng)	TaqMan		KASP		rhAmp
	Allele1	Allele2	Allele1	Allele2	Allele1	Allele2
100	1.5	2.3	0.7	1.1	5.9	3.4
90	1.4	2.2	0.6	0.5	5.9	3.5
80	1.6	2.2	0.6	0.6	5.9	3.6
70	1.6	2.3	0.6	0.6	5.9	3.6
60	1.6	2.4	0.6	0.5	6.1	3.7
50	1.6	2.4	0.7	0.6	6.1	3.6
40	1.6	2.3	0.7	0.6	6.1	3.6
30	1.5	2.3	0.7	0.8	5.9	3.4
20	1.4	2.2	0.8	0.6	5.3	2.5
10	1.4	2.3	0.6	0.6	1.6	1.9
1	0.6	1.7	0.4	0.3	2.8	1.4
0.9	0.9	1.4	0.3	0.4	1.5	1.6
0.8	0.9	1.7	0.2	0.2	2.3	0.9
0.7	0.9	1.6	0.2	0.3	1.5	0.7
0.6	0.7	1.4	0.2	0.3	1.0	1.1
0.5	0.4	1.6	0.2	0.1	1.1	1.2
0.4	0.8	0.9	0.1	0.2	1.5	0.9
0.3	0.5	1.4	0.1	0.1	1.6	0.9
0.2	0.9	0.7	0.1	0.1	0.8	1.0
0.1	0.1	0.8	0.1	0.1	0.1	0.1
Water	0.0	0.1	0.1	0.2	0.1	0.1

Each chemistry provides two ΔRn values, one for each allele. Samples above the level of detection (italics text) were genotyped. Samples below the level of detection (regular text) resulted undetermined