Fig. 4.

Effect of H₂O₂ on viral-potentiated IL-33 expression. Time course (a) or comparison at 4 and 24 h (b) of IL-33 expression after treatment with H₂O₂ and human rhinovirus, RV14 infection. NCI-H292 cells were infected with RV14 in the presence of H₂O₂ or vehicle. At various time points after incubation (a) or after 4 and 24 h (b), whole cells were harvested and assayed for the IL-33 gene expression. c Effect of NAC on H₂O₂-augmented IL-33 expression in RV14-treated cells. Cells were added with NAC before H₂O₂ treatment, and then infected with RV14. After 24 h RV14 infection, whole cells were harvested and assayed. Values are the mean ± SEM (n = 2 - 5). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the values of vehicle-treated cells