Fig. 1.

Evaluating the purity of primary cultured astrocytes and Cx43 expression in astrocytic subcellular components. Primary astrocytes were prepared from newborn mice. a Western blotting analyses of Cx43 levels were conducted with samples prepared from astrocytes from the control, OGD/R, OGD/R-SalB, and OGD/R-CBX groups. Representative protein bands are displayed for total protein extractions and the plasma membrane and cytoplasm compartments. b After OGD/R injury, Cx43 levels were significantly upregulated in the cytoplasm but downregulated in the plasma membrane. SalB treatment reversed these effects. The four groups did not significantly differ in total Cx43 levels. We evaluated the statistical significance with ANOVA and Duncan’s multiple comparisons test. *p < 0.05, **p < 0.01, and ***p < 0.001. Scale bar = 50 μm