Table 2.
PCR primers sequence used in this study.
| Name | Sequence (5′ to 3′) | |
|---|---|---|
| Semiquantitative PCR | ||
| iNOS | Forward | CCCTTCCGAAGTTTCTGGCAGCAG |
| Reverse | GGCTGTCAGAGCCTCGTGGCTTTGG | |
| COX-2 | Forward | CACTACATCCTGACCCACTT |
| Reverse | ATGCTCCTGCTTGAGTATGT | |
| TNF-α | Forward | TTGACCTCAGCGCTGAGTTG |
| Reverse | CCTGTAGCCCACGTCGTAGC | |
| GAPDH | Forward | CACTCACGGCAAATTCAACGGCA |
| Reverse | GACTCCACGACATACTCAGCAC | |
|
| ||
| Real-time PCR | ||
| iNOS | Forward | GGAGCCTTTAGACCTCAACAGA |
| Reverse | TGAACGAGGAGGGTGGTG | |
| COX-2 | Forward | CACTACATCCTGACCCACTT |
| Reverse | ATGCTCCTGCTTGAGTATGT | |
| TNF-α | Forward | GCCTCTTCTCATTCCTGCTTG |
| Reverse | CTGATGAGAGGGAGGCCATT | |
| IFN-β | Forward | AAGAGTTACACTGCCTTTGCCATC |
| Reverse | CACTGTCTGCTGGTGGAGTTCATC | |
| IFN-γ | Forward | GGGTTGTTGACCTCAAACTTGGCA |
| Reverse | CAGGCCATCAGCAACAACAT | |
| IL-1β | Forward | CAACCAACAAGTGATATTCTCCATG |
| Reverse | GATCCACACACTCCAGCTGCA | |
| IL-6 | Forward | CTAGGTTTGCCGAGTAGATCTC |
| Reverse | GACAAAGCCAGAGTCCTTCAGAGA | |
| IL-10 | Forward | ATAACTGCACCCACTTCCCA |
| Reverse | GGGCATCACTTCTACCAGGT | |
| TGF-β | Forward | AACAATTCCTGGCGTTACCTT |
| Reverse | CTGCCGTACAACTCCAGTGA | |
| GAPDH | Forward | CAATGAATACGGCTACAGCAAC |
| Reverse | AGGGAGATGCTCAGTGTTGG | |
COX-2, cyclooxygenase-2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; iNOS, inducible nitric oxide synthase; IFN-β, interferon-beta; IFN-γ, interferon-gamma; IL-1β, interleukin-1 beta; IL-6, interleukin 6; PCR, polymerase chain reaction; TNF-α, tumor necrosis factor-alpha.