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. 2018 Mar 13;2018:8968573. doi: 10.1155/2018/8968573

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Copyright © 2018 Xuehai Chen et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Measurement of the IOD/area of immunohistochemical staining of renal fibrogenic and interstitial fibrosis factors and apoptotic proteins. (a) Measurement of the IOD/area of α-SMA, ^∗∗p < 0.05. (b) Measurement of the IOD/area of caspase-3, ^∗p = 0.018 and ^∗∗∗p < 0.001. (c) Measurement of the IOD/area of TGF-β1, ^∗p < 0.05. IOD/area: integrated optical density/area. The mean of IOD/area of positive staining was used to accurately measure the degree of positive expression in immunohistochemistry samples.