Table 2.
Sequences of primers used for reverse transcription quantitative real-time PCR (RT-qPCR).
| Gene | Forward | Reverse |
|---|---|---|
| AFP1 | AGAAAACAGGGCGATGTCCA | TGCCTTGTCATACTGAGCGG |
| GPC32 | TAAAAGTCGCCCGTGTCGAA | ATGTAGCCTGGCAAAGCACT |
| HGF3 | CCCTATTTCCCGTTGTGAAGGA | ACCATCCACCCTACTGTTGTTT |
| TAT4 | GATTTTGGCAGTGGCTGAAAGG | GAACATTGGTGCTGAGGTTGG |
| ALB5 | AAGAGAAAGCACTGGTCGCA | GGGGAATCGCTGGCTCA-TAC |
| HNF-4a6 | AGGATGAAGAAGTTGCCCCC | GATGTGTCTGGTGGGTCCTG |
| CYP1A27 | CATCCTTTGTCCCCTTCACCA | GGTCTTTCCACTGCTTCTCATC |
| GAPDH8 | CTCTCTGCTCCTCCCTGTTC | TACGGCCAAATCCGTTCACA |
The sequences of primers (forward and reverse) used for reverse transcription quantitative real-time PCR (RT-qPCR). 1AFP: α-fetoprotein; 2GPC3: glypican 3; 3HGF: hepatocyte growth factor; 4TAT: tyrosine aminotransferase; 5ALB: albumin; 6HNF-4a: hepatocyte nuclear factor 4a; 7CYP1A2: cytochrome P450 1A2; 8GAPDH: glyceraldehyde 3-phosphate dehydrogenase (GAPDH was used as an endogenous control).