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. 2018 Mar 14;2018:1240192. doi: 10.1155/2018/1240192

Figure 7.

Figure 7

Lung fibrosis is increased in response to intermittent hypoxia. Quantitative RT-PCR was performed to estimate (a) Col1a1, (b) Col3a1, (c) Fn1, and (d) Ctgf mRNA levels in whole lung homogenate from mice in the 4 experimental groups and normalized to B2m mRNA. Results are expressed as means ± SE of 5 animals per group. (e) Lung histology of mice at day 21. Lung sections were prepared at day 21 in the 4 experimental groups and stained with haematoxylin and eosin to assess lung morphology. (f) Soluble collagen content was assessed by Sircol assay in lung homogenates in saline-treated mice (n = 5–10 mice per group) compared with BLM-treated mice (n = 14–18 mice per group). ∗p < 0.01 versus Saline-IA; ∗∗p < 0.01 versus Saline-IA; ∗∗∗p < 0.001 versus Saline-IA; #p < 0.05 versus BLM-IA; ##p < 0.01 versus BLM-IA.