Figure 7.

Serum IgG and intestinal IgA after prime-boost immunization using squalene-based vaccines containing ovalbumin alone or in combination with Mincle, Dectin-1, and Dectin-2 agonists. Mice were immunized twice by subcutaneous injection. Naïve mice were used as negative control. (a) Serum total IgG, (b) serum IgG1, IgG2a, IgG2b, and IgG3, and (c) intestinal IgA were quantified by ELISA 14 days after the second immunization. Data are individual log₂ antibody titers from one representative experiment (6 mice per group), with geometric mean and 95% confidence interval indicated. ^∗ p < 0.05; ^∗∗ p < 0.005 relative to control group by Mann–Whitney t-test.