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. 2018 Feb 26;10(3):264. doi: 10.3390/nu10030264

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of Pb exposure on the expression of 1-α-hydroxylase in the kidney of Wistar rat pups: (A) Western blot analysis of kidney tissue of control and Pb-exposed rats with 1-α-hydroxylase (CYP27B1) antibody at PND21 and PND30: 20 µg of kidney lysate was resolved on 10% SDS-PAGE and immunoblotted with antibody to CYP27B1. For loading control, the same membranes were stripped and re-probed with anti-actin antibody; (B) Quantification of Western blot bands at 32 kDa for CYP27B1 on the blots (shown in A): Blots (both CYP27B1 and actin) were scanned and the band densities quantified with Syngene gene tool software. Signal for this enzyme was normalized to signal for β-actin (for a loading control). Data are presented as Mean ± SD (n = 7). * Significantly different from control (p < 0.05).