Table 1.

List of Primary Antibodies.

Primary Antibody	Tissue	Colonality	Dilution	Source
Anti-CYP27B1 (1-α-hydroxylase)	Brain, kidney	polyclonal	1:1000 for WB 1:100 for IHC	USCN Life Sciences Inc., Wuhan, China
Anti-CYP27A1 (25-hydroxylase)	Liver	monoclonal	1:1000 for WB 1:100 for IHC	Abcam, Cambridge, MA, USA
VDR antibody	Brain	monoclonal	1:1000 for WB and IHC	Aviva systems biology, San Diego, CA, USA
Anti-β-actin	All the above tissues	monoclonal	1:2000	Sigma-Aldrich, St. Louis, MO, USA