Figure 2.

Representative time course of short circuit currents (I_sc) measured in Ussing chamber experiments. An increase in I_sc represents either transepithelial cation absorption or anion secretion. (a) protocol 1 (b) protocol 2. The black line represents one solvent control chamber while light blue and dark blue represent the low (10 µM) and high (300 µM) concentrations of RSV. Glucose and alanine were added mucosal (10 mM), while mannitol was added to the serosal compartment for osmotic reasons. Phlorizin (mucosal) and forskolin (serosal) were used at a final concentration of 0.01 mM.