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. 2018 Mar 15;10(3):356. doi: 10.3390/nu10030356

Figure 3.

Figure 3

Effect of KBH-JP-040 on pJNK and pp38 MAPK proteins, pIkBα, pNFkB in rhIL-1α-stimulated chondrocytes. The protein expression of pJNK and pp38MAP kinase IkBα, NFkB was analyzed by Western blot analysis. β-Actin was used as an internal control. Mock, normal chondrocytes; RhIL-1, 5 ng/mL recombinant human IL-1-treated group; RhIL-1+ KBH-JP-04050, rhIL-1 and standardized extract, KBH-JP-040 50 μg/mL-treated group; RhIL-1+ KBH-JP-04050, rhIL-1 and KBH-JP-040 50 μg/mL-treated group; RhIL-1+ KBH-JP-040100, rhIL-1- and KBH-JP-040 100 μg/mL-treated group; RhIL-1+ KBH-JP-04050, rhIL-1 and KBH-JP-040 150 μg/mL-treated group. The data are reported as means ± SD. **: p < 0.01; and ***: p < 0.001, Bonferroni post hoc test following one-way ANOVA versus the mock group; ##: p < 0.01; and ###: p < 0.001, Bonferroni post hoc test following one-way ANOVA versus RhIL-1 group. All experiments were performed using three replicates.