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. 2018 Mar 16;10(3):363. doi: 10.3390/nu10030363

Figure 2.

Figure 2

(A) Effects of coffee extracts with various roasting degrees on hepatic GSH concentration in LPS (15 mg/Kg.B.W/day)-injected C57BL/6 mice. Ten-week-old male C57BL/6 mice were pretreated with coffee extracts (300 mg/Kg.B.W/day) with four different roasting degrees for 10 days. mRNA expression levels of (B) GS, GCLC, GCLM, and (C) GPx were then analyzed by qRT-PCR and β-actin, housekeeping gene was used as an internal control. Different alphabets indicate significant differences (p < 0.05). Each bar represents the mean ± standard error (n = 6). GSH, glutathione; LPS, lipopolysaccharide; GS, glutathione synthase; GCLC, glutathione-cysteine ligase catalytic subunit; GCLM, glutathione-cysteine ligase modifier subunit; GPx, glutathione peroxidase; qRT-PCR, quantitative real-time reverse transcriptase-PCR.