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. 2018 Mar 16;10(3):363. doi: 10.3390/nu10030363

Figure 3.

Figure 3

Effects of coffee extracts with different roasting degrees on mRNA expression levels of genes related to pro-inflammatory cytokines in LPS (15 mg/Kg.B.W/day)-injected C57BL/6 mice. Ten-week-old male C57BL/6 mice were pretreated with coffee extracts (300 mg/Kg.B.W/day) of four different roasting degrees for 10 days. mRNA expression levels of (A) TNF-α, (B) iNOS, (C) IL-1β, (D) IL-6, (E) COX-2 were analyzed by qRT-PCR and β-actin was used as a housekeeping gene. Different alphabets indicate significant differences (p < 0.05). Each bar represents the mean ± standard error (n = 6). LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-alpha; iNOS, inducible nitric oxide synthase; IL-1β, interleukin 1-beta; IL-6, interleukin 6; COX-2, cyclooxygenase 2; qRT-PCR, quantitative real-time reverse transcriptase-PCR.