Fig. 2. The fluorescence response of the ONOO-CBP labeled RAW264.7 cells after incubation with different exogenous ROS and RNS for 60 minutes. (a) The live cell imaging of the ONOO-CBP labeled RAW264.7 cells in (i) the absence or (ii) the presence of 100 μM SIN-1 or (iii) 250 μM BA with 100 μM SIN-1. (b) The live cell imaging of the ONOO-CBP labeled RAW264.7 cells in the presence of (i) 50 μM SIN-1 or 100 μM each of (ii) NO, (iii) O2–, (iv) NO2–, (v) NO3–, (vi) HOCl, (vii) H2O2, or (viii) OH˙. The cell surface stained with streptavidin–Cy5 is shown in red and the nuclei labeled with Hoechst 34580 are shown in blue. Scale bar: 20 μm.
