Fig. 5. Coupling 3W-OSD or 3W-CHA to isothermal gene detection. (A) Scheme of the target gene. (B) Schematic of using 3W-OSD detection or 3W-CHA detection to probe loop amplicons of the LAMP reaction for the target gene. (C) Comparison of real-time 3W-CHA detection with real-time 3W-OSD detection during the LAMP reaction, with and without 2000 copies of the ompA gene template. 3W-OSD detector components added: TPompATT, OSD-Acceptor, OSD-F and OSD-Q. 3W-CHA detector components added: TP1ompATT, CHA1-H1ompA, CHA1-H2, CHA1-F and CHA1-Q.
