Fig. 7. One-tube one-channel detection of T1, T2 and T3. The concentrations of H1 were different for each target. Therefore, the existence and type for no target, a single target, two targets, and three targets could be distinguished by reading the final steady-state fluorescence of the CHA reaction. Whenever present, the concentration of T1, T2 or T3 was 5 nM.
