Fig. 8. One-tube one-channel detection of ompA and malB genes. (A) Sources of the ompA and malB genes. (B) Gel image of the LAMP amplicons amplified from no target, ompA, malB, and a mixture of both. (C) Responses of 3W-CHA to single ompA LAMP amplicons in (B). 3W-CHA detector components added: TP2ompATT, CHA2-H1ompA, CHA2-H2, CHA2-F and CHA2-Q. (D) Responses of 3W-CHA to single malB LAMP amplicons in (B). 3W-CHA detector components added: TP2malBTT, CHA2-H1malB, CHA2-H2, CHA2-F and CHA2-Q. (E) Multi-analysis responses of 3W-CHA to LAMP amplicons in (B). 3W-CHA detector components added: TP2ompATT, CHA2-H1ompA, TP2malBTT, CHA2-H1malB, CHA2-H2, CHA2-F and CHA2-Q. (F) Fluorescence images for the 3W-CHA reactions in (E) at 140 min.
