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. Author manuscript; available in PMC: 2018 Mar 28.
Published in final edited form as: Immunol Rev. 2011 Jan;239(1):178–196. doi: 10.1111/j.1600-065X.2010.00978.x

Fig. 4. The GLA-SE adjuvant promotes high quality Th1 responses characterized by the generation of antigen-specific pluripotent CD4+ T cells.

Fig. 4

Mice were immunized by injection of the Mycobacterium tuberculosis fusion antigens in the presence of GLA-SE. Spleens were collected, single cell suspensions prepared and cells cultured with antigen. (A) Cytokines secreted into the culture supernatant were measured by ELISA. (B) Cells were cultured with antigen and BD Golgi STOP (to prevent secretion) overnight, then fixed and stained for flow cytometry. Activated CD4+ T cells were identified based on CD3 and CD4 expression, and further gated as CD44hi. Graphs depict the proportion of activated CD4+ T cells expressing one, two, or the three cytokines (IFN-γ, TNF, and IL-2). Data originally published in (65).