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. Author manuscript; available in PMC: 2018 Mar 28.
Published in final edited form as: Immunol Rev. 2011 Jan;239(1):178–196. doi: 10.1111/j.1600-065X.2010.00978.x

Fig. 5. Use of various TLRL to generate IFNγ-producing antigen-specific CD4+ T cells.

Fig. 5

Mice were immunized by subcutaneous injection of the Mycobacterium leprae antigen ML0276 in the presence of CpG, IMQ, or GLA-SE at biweekly intervals, for a total of three immunizations. Spleens were collected 1 month after the third immunization, single cell suspensions prepared, and cells cultured with antigen and BD Golgi STOP (to prevent secretion) overnight, then fixed and stained for flow cytometry to determine the percentage CD3+CD4+CD44hi IFNγ cells. Data were originally published in (63).