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NMR can be used to characterize the HCA coating the bioactive MBG after being soaked in SBF. Top: The relative amount of ACP and HCA in the newly formed layer was quantified. NMR demonstrated that high Ca2+/PO43− ratios in the surrounding liquid medium retarded the HCA formation on MBG. Bottom: NMR demonstrated the effect of the MBG concentration used in the SBF tests.
