Figure 2.
The effects of FGF-1 and FGF-2 on the growth of the transfectants in serum-free culture. The cells (3 × 103 cells per well) were seeded in type I collagen-coated 24-well culture plates in RD5F and incubated with the indicated concentrations of FGF-1 or FGF-2 for 5 days. (A) FGF-1 at 20 ng/ml showed a 4-fold stimulation of HSYzeo growth (□) and a 2.5-fold stimulation of HSYR2/R1 growth (▵). (B) FGF-2 at 20 ng/ml showed a 4.8-fold stimulation of HSYzeo growth and a 2.5-fold stimulation of HSYR2/R1 growth. Neither FGF-1 nor FGF-2 stimulated the growth of HSYR2-IIIb (○). Mean values of duplicate wells from three independent experiments are shown.