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. 2001 Sep 25;98(20):11347–11352. doi: 10.1073/pnas.201418298

Figure 4.

Figure 4

Gas1 expression in the paraxial mesoderm is regulated by WNT and SHH. (A) E9.5 mouse psm explants were cultured in collagen gels. The inductive tissues were isolated from E2.5 chick. (Left to Right) psm was cultured alone, next to a notochord (nc), a ventral neural tube (vnt), a dorsal neural tube (dnt), or inside a surface ectoderm (se). Dashed lines, the boundary between psm and the chick tissues. Explants were assessed for Gas1 expression. (B) (Left to Right) psms were cocultured with control COS cells (COS-con) or cells expressing SHH-N (COS-SHH-N), BMP4/Furin (COS-BMP4/Furin), and WNT1 (COS-WNT1). Explants were assessed for Gas1 induction. Four samples are represented by each image. (C) Parental 3T3 cells (3T3-con) did not induce Gas1. WNT1-expressing cells (3T3-WNT1) induced Gas1 over 200 μm. The induction was reduced in the presence of 500 ng/ml SHH-N. WNT3A- and WNT4-expressing 3T3 cells also induced Gas1.