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. 2018 Mar 15;2(6):597–606. doi: 10.1182/bloodadvances.2017010975

Figure 4.

Figure 4.

Inhibitors effect on yield and injury of in vitro–grown megakaryocytes. (A) Yield of viable day 12 CD41+ megakaryocytes per HPC plated with or without the addition of SU6656, Y27632, and AZD1152. Mean yield ± 1 SEM is shown for N = 6 independent experiments. P values were determined using 1-way ANOVA in comparison with the control. (B) Yield of viable day 12 CD41+CD42bHigh megakaryocytes per HPC plated with or without the addition of SU6656, Y27632, and AZD1152 as in panel A. Mean yield ± 1 SEM is shown for N = 6 independent experiments. P values were determined using 1-way ANOVA in comparison with the control. (C) Percentage of viable CD41+ megakaryocytes that were also CD42bHigh on day 12 of culture. (D) Percentage of Annexin V+ CD41+CD42bHigh day 12 megakaryocytes was done as in panel B, but for Annexin binding to the megakaryocytes at day 12. For panels C and D, mean percentage-positive cells ± 1 SEM is shown for N ≥ 7 independent experiments. P values were determined using 1-way ANOVA in comparison with the control.