Figure 1.

Effects of egg white ovotransferrin‐derived tripeptides on cell viability and glucose uptake in L6 myotubes. A) Effects of IRW, IQW, or LKP on cell viability. Fully differentiated myotubes were incubated with 100 μm of IRW, IQW, or LKP 2 h prior to the treatment of 1 μm of Ang II for 24 h. Cell viability was examined using MTT assay. B) Effects of IRW, IQW, or LKP on insulin‐independent glucose uptake. Fully differentiated L6 myotubes were preincubated in 24‐place multiwell plates in Krebs‐Henseleit‐Hepes buffer (KHH buffer) without glucose for 2 h. They were then incubated in KHH buffer containing 11 mm glucose without or with insulin and 100 μm of peptides for 4 h. C) Effect of peptides on insulin stimulated glucose uptake in Ang II‐treated L6 myotubes. Fully differentiated myotubes were incubated with 100 μm of IRW, IQW, or LKP 2 h prior to the treatment of 1 μm of Ang II for 24 h. Next, the myotubes were kept for 2 h in KHH buffer. The myotubes were then cultured in KHH buffer containing 11 mm glucose in the absence or presence of 100 nm of insulin for another 4 h, and then the glucose uptake was measured using a Glucose CII Test Kit. Each value represents the mean ± SEM of five independent experiments. **indicates p < 0.05 as compared to the Ang II‐treated group. *indicates p < 0.05 as compared to the untreated group.