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. Author manuscript; available in PMC: 2018 Mar 28.
Published in final edited form as: Arch Pathol Lab Med. 2017 Mar;141(3):329–340. doi: 10.5858/arpa.2016-0277-SA

Figure 1.

Figure 1

The different methods utilized for molecular HLA typing include: A) Sequence Specific Priming (SSP) – PCR products amplified using sequence-specific primers are examined by agarose gel electrophoresis, which displays pattern of positive and negative reactions to determine presence or absence of HLA alleles. Each lane identifies a unique allele or set of alleles and the reaction pattern(s) is analyzed using HLA genotyping software. B) Sequence-specific oligonucleotide probing (SSOP) – PCR amplified products are added to specific oligonucleotide probes affixed to solid-phase matrices (e.g., a microparticle or microwell plate) and binding is assessed colorimetrically or via multiplex flow cytometry. C) Sequence-based testing (SBT) –nucleic acid sequencing of the HLA alleles are performed by Sanger or Next Generation (massively parallel) sequencing to determine the nucleotide sequence of HLA genotypes.