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. 2018 Feb 6;592(4):489–501. doi: 10.1002/1873-3468.12987

Figure 2.

Figure 2

Analytical ultracentrifugation (AUC) of GRAS domains. (A) The SCL5 GRAS domain exists as a monomer. The AUC sedimentation velocity analysis shows an estimated molecular mass of 46.6 ± 3.5 kDa, suggesting a monomer in solution (calculated mass 48.1 kDa). (B) The SCL3 GRAS domain exists as a homodimer. The analysis shows an estimated molecular mass of 106.3 ± 1.6 kDa, suggesting a homodimer in solution (calculated mass 50.8 kDa). (C) The SHR and SCR GRAS domains exist as a heterodimer. The analysis shows a monodispersed state with an estimated molecular mass of 105.4 ± 1.4 kDa, suggesting a heterodimer in solution (calculated mass 89.2 kDa).